Details of experiments between the fragments Q9BYF1~796-805 and Q92796~221-314
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ACE2
Protein A fragment: 796-805
Protein A site: PBM
Protein A sequence: QNTDDVQTSF
Protein A construct: Biotin-Ahx-QNTDDVQTSF
Protein B protein: DLG3
Protein B fragment: 221-314
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.76
Experiment series 2
Protein A protein: ACE2
Protein A fragment: 796-805
Protein A site: PBM
Protein A sequence: QNTDDVQTSF
Protein A construct: Biotin-Ahx-QNTDDVQTSF
Protein B protein: DLG3
Protein B fragment: 221-314
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.