Details of experiments between the fragments Q9BYB0~562-670 and Q9UDY2~1181-1190
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SHANK3
Protein A fragment: 562-670
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TJP2
Protein B fragment: 1181-1190
Protein B site: PBM
Protein B sequence: QSARYRDTEL
Protein B construct: biotin-ttds-QSARYRDTEL
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.