Details of experiments between Q9BYB0 and Q7Z6J2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: SHANK3
Protein A fragment: 562-670
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: GRASP
Protein B fragment: 386-395
Protein B site: PBM
Protein B sequence: RSLEEEESQL
Protein B construct: biotin-ttds-RSLEEEESQL

Average holdup BI: 0.57
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.24