Details of experiments between Q9BYB0 and P03409
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: SHANK3
Protein A fragment: 562-670
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV

Average holdup BI: -0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.