Details of experiments between the fragments Q9BW19~358-372 and P00519~56-121
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KIFC1
Protein A fragment: 358-372
Protein A site: PxxP_2
Protein A sequence: GTLSGAPAPPTRHDF
Protein A construct: Bio Ado Ado-GTLSGAPAPPTRHDF-CONH2
Protein B protein: ABL1
Protein B fragment: 56-121
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.