Details of experiments between Q9BSE5 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: AGMAT
Protein A fragment: 343-352
Protein A site: PBM
Protein A sequence: LCALPKVTTV
Protein A construct: biotin-ttds-LCALPKVTTV
Protein B protein: NHERF3
Protein B fragment: 375-459
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.51
Experiment series 2
Protein A protein: AGMAT
Protein A fragment: 343-352
Protein A site: PBM
Protein A sequence: LCALPKVTTV
Protein A construct: biotin-ttds-LCALPKVTTV
Protein B protein: NHERF3
Protein B fragment: 2-107
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: AGMAT
Protein A fragment: 343-352
Protein A site: PBM
Protein A sequence: LCALPKVTTV
Protein A construct: biotin-ttds-LCALPKVTTV
Protein B protein: NHERF3
Protein B fragment: 238-329
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.