Details of experiments between the fragments Q9BRD0~194-208 and P49418~615-695
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: BUD13
Protein A fragment: 194-208
Protein A site: PxxP_1
Protein A sequence: RHDSPDPSPPRRPQH
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 3.68