Details of experiments between the fragments Q9BQQ3~1-208 and O14745~349-358
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GORASP1
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: NHERF1
Protein B fragment: 349-358
Protein B site: PBM
Protein B sequence: SKKNELFSNL
Protein B construct: Biotin-Ahx-SKKNELFSNL
Average holdup BI: -0.04
Normalized holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.