Details of experiments between Q9BQQ3 and Q9UDY2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GORASP1
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TJP2
Protein B fragment: 1181-1190
Protein B site: PBM
Protein B sequence: QSARYRDTEL
Protein B construct: biotin-ttds-QSARYRDTEL
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: GORASP1
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TJP2
Protein B fragment: 1181-1190
Protein B site: PBM
Protein B sequence: QSARYRDTEL
Protein B construct: biotin-ttds-QSARYRDTEL
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.