Details of experiments between Q99767 and Q9IR59
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: APBA2
Protein A fragment: 565-653
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: APBA2
Protein A fragment: 655-735
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.09
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: APBA2
Protein A fragment: 565-653
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.02
Holdup BI standard deviation: 0.03
Normalized holdup BI: -0.02
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: APBA2
Protein A fragment: 655-735
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.02
Holdup BI standard deviation: 0
Normalized holdup BI: -0.02
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.