Details of experiments between Q99767 and Q9C0A0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: APBA2
Protein A fragment: 565-653
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CNTNAP4
Protein B fragment: 1299-1308
Protein B site: PBM
Protein B sequence: VNENQKEYFF
Protein B construct: Biotin-Ahx-VNENQKEYFF
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: APBA2
Protein A fragment: 655-735
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CNTNAP4
Protein B fragment: 1299-1308
Protein B site: PBM
Protein B sequence: VNENQKEYFF
Protein B construct: Biotin-Ahx-VNENQKEYFF
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.