Details of experiments between the fragments Q99501~326-340 and Q14155~178-251
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GAS2L1
Protein A fragment: 326-340
Protein A site: PxxP_3
Protein A sequence: RSTKEGPETPPRPRD
Protein A construct: Bio Ado Ado-RSTKEGPETPPRPRD-CONH2
Protein B protein: ARHGEF7
Protein B fragment: 178-251
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
Measured pKd: 3.72