Details of experiments between the fragments Q96RT1~1314-1412 and Q14155~794-803
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LAP2
Protein A fragment: 1314-1412
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGEF7
Protein B fragment: 794-803
Protein B site: PBM
Protein B sequence: NDPAWDETNL
Protein B construct: biotin-ttds-NDPAWDETNL
Average holdup BI: 0.28
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.33
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 4.46