Details of experiments between Q96QZ7 and Q9ULW2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI1
Protein A fragment: 456-580
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FZD10
Protein B fragment: 572-581
Protein B site: PBM
Protein B sequence: EIPAQSPTCV
Protein B construct: biotin-ado-ado-EIPAQSPTCV
Average holdup BI: 0.11
Normalized holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.88
Experiment series 2
Protein A protein: MAGI1
Protein A fragment: 1-106
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FZD10
Protein B fragment: 572-581
Protein B site: PBM
Protein B sequence: EIPAQSPTCV
Protein B construct: biotin-ado-ado-EIPAQSPTCV
Average holdup BI: 0.1
Normalized holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.79