Details of experiments between Q96QZ7 and Q9HCD6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI1
Protein A fragment: 1-106
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TANC2
Protein B fragment: 1981-1990
Protein B site: PBM
Protein B sequence: PKRPFVESNV
Protein B construct: biotin-ttds-PKRPFVESNV
Average holdup BI: -0.07
Holdup BI standard deviation: 0.1
Normalized holdup BI: -0.07
Normalized holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: MAGI1
Protein A fragment: 456-580
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TANC2
Protein B fragment: 1981-1990
Protein B site: PBM
Protein B sequence: PKRPFVESNV
Protein B construct: biotin-ttds-PKRPFVESNV
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.