Details of experiments between the fragments Q96L92~1-541 and Q13574~919-928
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNX27
Protein A fragment: 1-541
Protein A construct: endogenous protein (Jurkat)
Protein B protein: DGKZ
Protein B fragment: 919-928
Protein B site: PBM
Protein B sequence: IQREDQETAV
Protein B construct: biotin-ttds-IQREDQETAV
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation (8 point titration)
Date of measurement: 2022.08.03.
PUBMED ID of publication: 36542723
Number of measurements: 3
Measured pKd: 6.4
Experiment series 2
Protein A protein: SNX27
Protein A fragment: 1-541
Protein A construct: endogenous protein (Jurkat)
Protein B protein: DGKZ
Protein B fragment: 919-928
Protein B site: PBM
Protein B sequence: IQREDQETAV
Protein B construct: biotin-ttds-IQREDQETAV
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.08.03.
PUBMED ID of publication: 36542723
Number of measurements: 3
Measured pKd: 6.03