Details of experiments between the fragments Q96L92~1-541 and P08670~1-466
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNX27
Protein A fragment: 1-541
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SNX27
Protein B protein: VIM
Protein B fragment: 1-466
Protein B construct: endogenous protein (Jurkat)
Average holdup BI: 0.95
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 12.82
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.27.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 6))
PUBMED ID of publication: 36542723
Number of measurements: 6
Measured pKd: 6.32
Experiment series 2
Protein A protein: SNX27
Protein A fragment: 1-541
Protein A construct: avi(biotin)-MBP-SNX27-his6
Protein B protein: VIM
Protein B fragment: 1-466
Protein B construct: endogenous protein (Jurkat)
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: Hill equation (8 point titration) h=1.9
Date of measurement: 2022.08.03.
PUBMED ID of publication: 36542723
Number of measurements: 3
Measured pKd: 7.1