Details of experiments between Q96L92 and Q81964
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNX27
Protein A fragment: 40-141
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV59-E6
Protein B fragment: 151-160
Protein B site: PBM
Protein B sequence: QRQARSETLV
Protein B construct: biotin-ttds-QRQARSETLV
Average holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.62
Experiment series 2
Protein A protein: SNX27
Protein A fragment: 40-141
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV59-E6
Protein B fragment: 151-160
Protein B site: PBM
Protein B sequence: QRQARSETLV
Protein B construct: biotin-ttds-QRQARSETLV
Average holdup BI: 0.35
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.35
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 6
Measured pKd: 4.64