Details of experiments between Q96L92 and P46095
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNX27
Protein A fragment: 40-141
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: GPR6
Protein B fragment: 353-362
Protein B site: PBM
Protein B sequence: PFRSRSPSEV
Protein B construct: biotin-ttds-PFRSRSPSEV
Average holdup BI: 0.02
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 3
The affinity was below the detection threshold of the assay.