Details of experiments between Q96L92 and O95049
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNX27
Protein A fragment: 40-141
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TJP3
Protein B fragment: 910-919
Protein B site: PBM
Protein B sequence: GYDWGPATDL
Protein B construct: Biotin-Ahx-GYDWGPATDL
Average holdup BI: 0.06
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.64