Details of experiments between the fragments Q96KB5~313-322 and O15018~326-424
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: PDZD2
Protein B fragment: 326-424
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.06
Experiment series 2
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: PDZD2
Protein B fragment: 326-424
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.41
Normalized holdup BI: 0.41
Immobilized partner concentration in holdup experiment (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.84