Details of experiments between Q96KB5 and O75970
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 120-230
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1624-1716
Protein B site: PDZ_10
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1722-1810
Protein B site: PDZ_11
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1858-1949
Protein B site: PDZ_12
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1982-2070
Protein B site: PDZ_13
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 246-342
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.15
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 371-467
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 548-634
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.06
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 688-793
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 998-1105
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1141-1246
Protein B site: PDZ_7
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1325-1441
Protein B site: PDZ_8
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1464-1572
Protein B site: PDZ_9
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1624-1716
Protein B site: PDZ_10
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 1858-1949
Protein B site: PDZ_12
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 16
Protein A protein: PBK
Protein A fragment: 313-322
Protein A site: PBM
Protein A sequence: HIVEALETDV
Protein A construct: biotin-ttds-HIVEALETDV
Protein B protein: MPDZ
Protein B fragment: 688-793
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.