Details of experiments between Q96JH8 and Q9Y6N9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: RADIL
Protein A fragment: 973-1075
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: USH1C
Protein B fragment: 543-552
Protein B site: PBM
Protein B sequence: KEYDDELTFF
Protein B construct: Biotin-Ahx-KEYDDELTFF

Average holdup BI: 0.1
Normalized holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.78