Details of experiments between Q96JH8 and Q9Y597
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RADIL
Protein A fragment: 973-1075
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCTD3
Protein B fragment: 806-815
Protein B site: PBM
Protein B sequence: SDSSGQEYSL
Protein B construct: Biotin-Ahx-SDSSGQEYSL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.