Details of experiments between the fragments Q96FS4~683-764 and Q9Q6P4~3424-3433
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SIPA1
Protein A fragment: 683-764
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: -0.03
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 3
The affinity was below the detection threshold of the assay.