Details of experiments between the fragments Q96FS4~683-764 and Q8N743~401-410
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SIPA1
Protein A fragment: 683-764
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KIR3DL3
Protein B fragment: 401-410
Protein B site: PBM
Protein B sequence: PKTPPTDTSV
Protein B construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.