Details of experiments between the fragments Q96DX4~71-85 and P49418~615-695
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: RSPRY1
Protein A fragment: 71-85
Protein A site: PxxP_1
Protein A sequence: ADTRSQPRDPVRPPR
Protein A construct: Bio Ado Ado-ADTRSQPRDPVRPPR-CONH2

Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.66
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
Measured pKd: 5.09