Details of experiments between the fragments Q969H4~194-283 and P36507~391-400
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CNKSR1
Protein A fragment: 194-283
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: MAP2K2
Protein B fragment: 391-400
Protein B site: PBM
Protein B sequence: QPGTPTRTAV
Protein B construct: biotin-ttds-QPGTPTRTAV
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.07