Details of experiments between the fragments Q92796~381-476 and P23634~1232-1241
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG3
Protein A fragment: 381-476
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.39
Experiment series 2
Protein A protein: DLG3
Protein A fragment: 381-476
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: 0.37
Normalized holdup BI: 0.46
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.73
Experiment series 3
Protein A protein: DLG3
Protein A fragment: 381-476
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: 0.32
Normalized holdup BI: 0.37
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.56