Details of experiments between Q92796 and Q8N103
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG3
Protein A fragment: 221-314
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TAGAP
Protein B fragment: 722-731
Protein B site: PBM
Protein B sequence: QFQYAKESYI
Protein B construct: biotin-ttds-QFQYAKESYI
Average holdup BI: 0.16
Normalized holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.15
Experiment series 2
Protein A protein: DLG3
Protein A fragment: 381-476
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TAGAP
Protein B fragment: 722-731
Protein B site: PBM
Protein B sequence: QFQYAKESYI
Protein B construct: biotin-ttds-QFQYAKESYI
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.57
Experiment series 3
Protein A protein: DLG3
Protein A fragment: 124-219
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TAGAP
Protein B fragment: 722-731
Protein B site: PBM
Protein B sequence: QFQYAKESYI
Protein B construct: biotin-ttds-QFQYAKESYI
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.