Details of experiments between Q92796 and Q8IW70
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG3
Protein A fragment: 221-314
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TMEM151B
Protein B fragment: 557-566
Protein B site: PBM
Protein B sequence: RHGSCVETSL
Protein B construct: biotin-ado-ado-RHGSCVETSL
Average holdup BI: 0.34
Normalized holdup BI: 0.36
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.53
Experiment series 2
Protein A protein: DLG3
Protein A fragment: 381-476
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TMEM151B
Protein B fragment: 557-566
Protein B site: PBM
Protein B sequence: RHGSCVETSL
Protein B construct: biotin-ado-ado-RHGSCVETSL
Average holdup BI: 0.18
Normalized holdup BI: 0.22
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.2
Experiment series 3
Protein A protein: DLG3
Protein A fragment: 124-219
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TMEM151B
Protein B fragment: 557-566
Protein B site: PBM
Protein B sequence: RHGSCVETSL
Protein B construct: biotin-ado-ado-RHGSCVETSL
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.53