Details of experiments between the fragments Q92793~2433-2442 and O96018~482-569
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CBP
Protein A fragment: 2433-2442
Protein A site: PBM
Protein A sequence: DTLEKFVEGL
Protein A construct: Biotin-ttds-TGDTLEKFVEGL
Protein B protein: APBA3
Protein B fragment: 482-569
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.95