Details of experiments between Q92793 and Q92997
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CBP
Protein A fragment: 2433-2442
Protein A site: PBM
Protein A sequence: DTLEKFVEGL
Protein A construct: Biotin-ttds-TGDTLEKFVEGL
Protein B protein: DVL3
Protein B fragment: 244-343
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: CBP
Protein A fragment: 2433-2442
Protein A site: PBM
Protein A sequence: DTLEKFVEGL
Protein A construct: Biotin-ttds-TGDTLEKFVEGL
Protein B protein: DVL3
Protein B fragment: 244-343
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.