Details of experiments between the fragments Q92743~365-480 and Q9Y4G8~1490-1499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HTRA1
Protein A fragment: 365-480
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RAPGEF2
Protein B fragment: 1490-1499
Protein B site: PBM
Protein B sequence: EDEDEQVSAV
Protein B construct: Biotin-Ahx-EDEDEQVSAV
Average holdup BI: 0.16
Holdup BI standard deviation: 0
Normalized holdup BI: 0.2
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.18