Details of experiments between the fragments Q92743~365-480 and Q15418~683-735
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HTRA1
Protein A fragment: 365-480
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RPS6KA1
Protein B fragment: 683-735
Protein B site: PBM
Protein B sequence: RVRKLPSTTL
Protein B construct: ...KLPSTTL
Measured dissociation constant (10-6M): 19
Standard deviation of dissociation constant (10-6M): 3
Experiment method: FP_competitive
PUBMED ID of publication: 30726710
Number of measurements: 3
Measured pKd: 4.72
Experiment series 2
Protein A protein: HTRA1
Protein A fragment: 365-480
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RPS6KA1
Protein B fragment: 683-735
Protein B site: PBM
Protein B modification: (SEP)732
Protein B sequence: RVRKLPSTTL
Protein B construct: ...KLP(pS)TTL
Measured dissociation constant (10-6M): 33
Standard deviation of dissociation constant (10-6M): 2
Experiment method: FP_competitive
PUBMED ID of publication: 30726710
Number of measurements: 3
Measured pKd: 4.48