Details of experiments between the fragments Q92619~1106-1120 and Q14155~178-251
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGAP45
Protein A fragment: 1106-1120
Protein A site: PxxP_1
Protein A sequence: NNVLQAPLPPMRLRG
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: ARHGEF7
Protein B fragment: 178-251
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.2
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 4.17
Experiment series 2
Protein A protein: ARHGAP45
Protein A fragment: 1106-1120
Protein A site: PxxP_1
Protein A sequence: NNVLQAPLPPMRLRG
Protein A construct: Bio E E Ado Ado-NNVLQAPLPPMRLRG-CONH2
Protein B protein: ARHGEF7
Protein B fragment: 178-251
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.13