Details of experiments between Q8WXI2 and Q8TEW0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CNKSR2
Protein A fragment: 1025-1034
Protein A site: PBM
Protein A sequence: HTHSYIETHV
Protein A construct: Biotin-Ahx-HTHSYIETHV
Protein B protein: PARD3
Protein B fragment: 582-685
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.