Details of experiments between Q8WXI2 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CNKSR2
Protein A fragment: 1025-1034
Protein A site: PBM
Protein A sequence: HTHSYIETHV
Protein A construct: Biotin-Ahx-HTHSYIETHV
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.36
Normalized holdup BI: 0.36
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.54
Experiment series 2
Protein A protein: CNKSR2
Protein A fragment: 1025-1034
Protein A site: PBM
Protein A sequence: HTHSYIETHV
Protein A construct: Biotin-Ahx-HTHSYIETHV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.28
Normalized holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.42