Details of experiments between Q8WWN8 and Q92796
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARAP3
Protein A fragment: 1417-1431
Protein A site: Internal_PBM
Protein A construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Protein B protein: DLG3
Protein B fragment: 221-314
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Normalized holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.16
Experiment series 2
Protein A protein: ARAP3
Protein A fragment: 1417-1431
Protein A site: Internal_PBM
Protein A construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Protein B protein: DLG3
Protein B fragment: 124-219
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: ARAP3
Protein A fragment: 1417-1431
Protein A site: Internal_PBM
Protein A construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Protein B protein: DLG3
Protein B fragment: 381-476
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.