Details of experiments between Q8WWN8 and Q68DX3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARAP3
Protein A fragment: 1417-1431
Protein A site: Internal_PBM
Protein A construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Protein B protein: FRMPD2
Protein B fragment: 770-861
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: ARAP3
Protein A fragment: 1417-1431
Protein A site: Internal_PBM
Protein A construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Protein B protein: FRMPD2
Protein B fragment: 944-1038
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: ARAP3
Protein A fragment: 1417-1431
Protein A site: Internal_PBM
Protein A construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Protein B protein: FRMPD2
Protein B fragment: 1073-1166
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.