Details of experiments between the fragments Q8WUI4~943-952 and Q6P0Q8~1102-1193
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HDAC7
Protein A fragment: 943-952
Protein A site: PBM
Protein A sequence: LVEEEEPMNL
Protein A construct: biotin-ado-ado-LVEEEEPMNL
Protein B protein: MAST2
Protein B fragment: 1102-1193
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.08