Details of experiments between Q8TF72 and Q9Y3R0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SHROOM3
Protein A fragment: 1987-1996
Protein A site: PBM
Protein A sequence: GIFPTLTSPL
Protein A construct: Biotin-Ahx-GIFPTLTSPL
Protein B protein: GRIP1
Protein B fragment: 242-344
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: SHROOM3
Protein A fragment: 1987-1996
Protein A site: PBM
Protein A sequence: GIFPTLTSPL
Protein A construct: Biotin-Ahx-GIFPTLTSPL
Protein B protein: GRIP1
Protein B fragment: 464-567
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: SHROOM3
Protein A fragment: 1987-1996
Protein A site: PBM
Protein A sequence: GIFPTLTSPL
Protein A construct: Biotin-Ahx-GIFPTLTSPL
Protein B protein: GRIP1
Protein B fragment: 563-659
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: SHROOM3
Protein A fragment: 1987-1996
Protein A site: PBM
Protein A sequence: GIFPTLTSPL
Protein A construct: Biotin-Ahx-GIFPTLTSPL
Protein B protein: GRIP1
Protein B fragment: 996-1086
Protein B site: PDZ_7
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.