Details of experiments between Q8TEW0 and P62258
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: PARD3
Protein A fragment: 884-893
Protein A site: 14-3-3_pSer889
Protein A modification: (SEP)889
Protein A sequence: LKKSSSLESL
Protein A construct: LKKSS(pSer)LESL (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.29
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.47


Experiment series 2

Protein A protein: PARD3
Protein A fragment: 1218-1227
Protein A site: 14-3-3_pSer1223
Protein A modification: (SEP)1223
Protein A sequence: QRQYSSLPRQ
Protein A construct: QRQYS(pSer)LPRQ (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.76
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.77
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.34


Experiment series 3

Protein A protein: PARD3
Protein A fragment: 1218-1227
Protein A site: 14-3-3_pSer1223
Protein A modification: (SEP)1223
Protein A sequence: QRQYSSLPRQ
Protein A construct: QRQYS(pSer)LPRQ (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.64
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 4.19


Experiment series 4

Protein A protein: PARD3
Protein A fragment: 884-893
Protein A site: 14-3-3_pSer889
Protein A modification: (SEP)889
Protein A sequence: LKKSSSLESL
Protein A construct: LKKSS(pSer)LESL (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.24
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 5

Protein A protein: PARD3
Protein A fragment: 293-302
Protein A site: 14-3-3_pThr298
Protein A modification: (TPO)298
Protein A sequence: ARGGRTLGLL
Protein A construct: ARGGR(pThr)LGLL (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.46
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.77


Experiment series 6

Protein A protein: PARD3
Protein A fragment: 293-302
Protein A site: 14-3-3_pThr298
Protein A modification: (TPO)298
Protein A sequence: ARGGRTLGLL
Protein A construct: ARGGR(pThr)LGLL (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.31
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.59


Experiment series 7

Protein A protein: PARD3
Protein A fragment: 1351-1360
Protein A site: 14-3-3_pSer1356
Protein A modification: (SEP)1356
Protein A sequence: GRPFYS----
Protein A construct: TPEKGRPFY(pSer) (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.15
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 3.45
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 8

Protein A protein: PARD3
Protein A fragment: 1351-1360
Protein A site: 14-3-3_pSer1356
Protein A modification: (SEP)1356
Protein A sequence: GRPFYS----
Protein A construct: TPEKGRPFY(pSer) (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.16
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 3.25
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.13


Experiment series 9

Protein A protein: PARD3
Protein A fragment: 476-485
Protein A site: 14-3-3_pThr481
Protein A modification: (TPO)481
Protein A sequence: TSRDVTIGGS
Protein A construct: TSRDV(pThr)IGGS (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.05
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 10

Protein A protein: PARD3
Protein A fragment: 171-180
Protein A site: 14-3-3_pThr176
Protein A modification: (TPO)176
Protein A sequence: TRWSTTAGFL
Protein A construct: TRWST(pThr)AGFL (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.36
Holdup BI standard deviation: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.59


Experiment series 11

Protein A protein: PARD3
Protein A fragment: 822-831
Protein A site: 14-3-3_pSer827
Protein A modification: (SEP)827
Protein A sequence: GFGRQSMSEK
Protein A construct: GFGRQ(pSer)MSEK (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.1
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 12

Protein A protein: PARD3
Protein A fragment: 710-719
Protein A site: 14-3-3_pSer715
Protein A modification: (SEP)715
Protein A sequence: RERRISHSLY
Protein A construct: RERRI(pSer)HSLY (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: -0.33
Holdup BI standard deviation: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.72
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 13

Protein A protein: PARD3
Protein A fragment: 968-977
Protein A site: 14-3-3_pSer973
Protein A modification: (SEP)973
Protein A sequence: DQPSHSLERQ
Protein A construct: DQPSH(pSer)LERQ (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: -0.26
Holdup BI standard deviation: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 14

Protein A protein: PARD3
Protein A fragment: 1332-1341
Protein A site: 14-3-3_pSer1337
Protein A modification: (SEP)1337
Protein A sequence: VPPSPSQVAR
Protein A construct: VPPSP(pSer)QVAR (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.03
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 15

Protein A protein: PARD3
Protein A fragment: 822-831
Protein A site: 14-3-3_pSer827
Protein A modification: (SEP)827
Protein A sequence: GFGRQSMSEK
Protein A construct: GFGRQ(pSer)MSEK (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.21
Holdup BI standard deviation: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 16

Protein A protein: PARD3
Protein A fragment: 216-225
Protein A site: 14-3-3_pSer221
Protein A modification: (SEP)221
Protein A sequence: DNARSSLSAS
Protein A construct: DNARS(pSer)LSAS (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.25
Holdup BI standard deviation: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.9
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 17

Protein A protein: PARD3
Protein A fragment: 876-885
Protein A site: 14-3-3_pSer881
Protein A modification: (SEP)881
Protein A sequence: RDVGPSLGLK
Protein A construct: RDVGP(pSer)LGLK (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.04
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.89
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 18

Protein A protein: PARD3
Protein A fragment: 196-205
Protein A site: 14-3-3_pSer201
Protein A modification: (SEP)201
Protein A sequence: DENYRSLPRD
Protein A construct: DENYR(pSer)LPRD (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.05
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.84
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 19

Protein A protein: PARD3
Protein A fragment: 196-205
Protein A site: 14-3-3_pSer201
Protein A modification: (SEP)201
Protein A sequence: DENYRSLPRD
Protein A construct: DENYR(pSer)LPRD (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.06
Holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.81
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 20

Protein A protein: PARD3
Protein A fragment: 171-180
Protein A site: 14-3-3_pThr176
Protein A modification: (TPO)176
Protein A sequence: TRWSTTAGFL
Protein A construct: TRWST(pThr)AGFL (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.17
Holdup BI standard deviation: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.7
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 21

Protein A protein: PARD3
Protein A fragment: 20-29
Protein A site: 14-3-3_pSer25
Protein A modification: (SEP)25
Protein A sequence: HMKVFSLIQQ
Protein A construct: HMKVF(pSer)LIQQ (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: -0.41
Holdup BI standard deviation: 0.48
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.54
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 22

Protein A protein: PARD3
Protein A fragment: 715-724
Protein A site: 14-3-3_pSer720
Protein A modification: (SEP)720
Protein A sequence: SHSLYSGIEG
Protein A construct: SHSLY(pSer)GIEG (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: -0.03
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.29
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 23

Protein A protein: PARD3
Protein A fragment: 715-724
Protein A site: 14-3-3_pSer720
Protein A modification: (SEP)720
Protein A sequence: SHSLYSGIEG
Protein A construct: SHSLY(pSer)GIEG (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: -0.04
Holdup BI standard deviation: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 24

Protein A protein: PARD3
Protein A fragment: 139-148
Protein A site: 14-3-3_pSer144
Protein A modification: (SEP)144
Protein A sequence: HVRRSSDPAL
Protein A construct: HVRRS(pSer)DPAL (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: -0.2
Holdup BI standard deviation: 0.66
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 25

Protein A protein: PARD3
Protein A fragment: 1332-1341
Protein A site: 14-3-3_pSer1337
Protein A modification: (SEP)1337
Protein A sequence: VPPSPSQVAR
Protein A construct: VPPSP(pSer)QVAR (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.02
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 26

Protein A protein: PARD3
Protein A fragment: 968-977
Protein A site: 14-3-3_pSer973
Protein A modification: (SEP)973
Protein A sequence: DQPSHSLERQ
Protein A construct: DQPSH(pSer)LERQ (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: -0.01
Holdup BI standard deviation: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 27

Protein A protein: PARD3
Protein A fragment: 476-485
Protein A site: 14-3-3_pThr481
Protein A modification: (TPO)481
Protein A sequence: TSRDVTIGGS
Protein A construct: TSRDV(pThr)IGGS (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 28

Protein A protein: PARD3
Protein A fragment: 216-225
Protein A site: 14-3-3_pSer221
Protein A modification: (SEP)221
Protein A sequence: DNARSSLSAS
Protein A construct: DNARS(pSer)LSAS (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.02
Holdup BI standard deviation: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 29

Protein A protein: PARD3
Protein A fragment: 1173-1182
Protein A site: 14-3-3_pSer1178
Protein A modification: (SEP)1178
Protein A sequence: RRRTYSFEQP
Protein A construct: RRRTY(pSer)FEQP (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0.03
Holdup BI standard deviation: 0.47
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 30

Protein A protein: PARD3
Protein A fragment: 876-885
Protein A site: 14-3-3_pSer881
Protein A modification: (SEP)881
Protein A sequence: RDVGPSLGLK
Protein A construct: RDVGP(pSer)LGLK (crude synthetic peptide)

Protein B protein: YWHAE
Protein B fragment: 1-255
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Average holdup BI: 0
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.