Details of experiments between the fragments Q8TEU7~525-611 and Q82005~139-148
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RAPGEF6
Protein A fragment: 525-611
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV73-E6
Protein B fragment: 139-148
Protein B site: PBM
Protein B sequence: SGSRPSATVV
Protein B construct: biotin-ttds-SGSRPSATVV
Average holdup BI: -0.24
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: RAPGEF6
Protein A fragment: 525-611
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV73-E6
Protein B fragment: 139-148
Protein B site: PBM
Protein B sequence: SGSRPSATVV
Protein B construct: biotin-ttds-SGSRPSATVV
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.57