Details of experiments between the fragments Q8TEU7~525-611 and Q68D42~226-235
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RAPGEF6
Protein A fragment: 525-611
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TMEM215
Protein B fragment: 226-235
Protein B site: PBM
Protein B sequence: QGRWDHETIV
Protein B construct: Biotin-Ahx-QGRWDHETIV
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.