Details of experiments between the fragments Q8TEU7~525-611 and Q12933~492-501
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RAPGEF6
Protein A fragment: 525-611
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TRAF2
Protein B fragment: 492-501
Protein B site: PBM
Protein B sequence: IKAIVDLTGL
Protein B construct: biotin-ado-ado-IKAIVDLTGL
Average holdup BI: -0.07
Normalized holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.