Details of experiments between the fragments Q8TDW7~4548-4557 and O43464~355-458
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FAT3
Protein A fragment: 4548-4557
Protein A site: PBM
Protein A sequence: FVETQHQTQV
Protein A construct: biotin-ttds-FVETQHQTQV
Protein B protein: HTRA2
Protein B fragment: 355-458
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.06
Holdup BI standard deviation: 0.12
Normalized holdup BI: -0.06
Normalized holdup BI standard deviation: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.