Details of experiments between the fragments Q8TBC3~698-707 and Q92743~365-480
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SHKBP1
Protein A fragment: 698-707
Protein A site: PBM
Protein A sequence: PKMKLNETSF
Protein A construct: Biotin-Ahx-PKMKLNETSF
Protein B protein: HTRA1
Protein B fragment: 365-480
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Holdup BI standard deviation: 0.01
Normalized holdup BI: -0.04
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.