Details of experiments between Q8TBB1 and Q9Q6P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LNX1
Protein A fragment: 261-371
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.1
Holdup BI standard deviation: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: LNX1
Protein A fragment: 371-470
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: -0
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: LNX1
Protein A fragment: 500-596
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: -0.04
Holdup BI standard deviation: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: LNX1
Protein A fragment: 631-726
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: -0.07
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: LNX1
Protein A fragment: 261-371
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.06
Normalized holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.74
Experiment series 6
Protein A protein: LNX1
Protein A fragment: 371-470
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: LNX1
Protein A fragment: 500-596
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.