Details of experiments between Q8TAA9 and Q8TEU7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: VANGL1
Protein A fragment: 515-524
Protein A site: PBM
Protein A sequence: VLRLQSETSV
Protein A construct: biotin-ttds-VLRLQSETSV
Protein B protein: RAPGEF6
Protein B fragment: 525-611
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.